Megakaryoblasts And Erythroblasts In Mice Bone Marrow After Gamma-irradiation With Sublethal Doses. Extracellular Ubiquitin Effect

Abstract

Studies show that erythropoiesis and thrombopoiesis are interrelated. We provided cytological and statistical analyses of elevation-depletion picks for megakaryocytes and erythroblasts in bone marrow (BM) and the passage of mature cells into the bloodstream (PB) of irradiated mice. The source of radiation was 137Cs with dose rate 1Gy/min., due exposure 5min. Nonlinear white mice of 23±2gr. were used for tests. Animals were divided into three groups: the first control group of intact mice; the second test group of mice irradiated with the dose of LD50 5Gy; the third test group of mice irradiated with 5Gy intraperitoneally injected with ubiquitin at the 72-hour point after irradiation. PB and BM samples from the control group and the test groups of mice have been taken every 24 hours after irradiation for 7 days. Microscopy and statistical methods have been used for calculation of cell count of PB and BM.

Analysis of the most active periods of bone marrow spontaneous regeneration - 3-5th days – showed us that intraperitoneally injected ubiquitin modulates the ratio of erythroblast/megakaryocytesand descends pick sizes during proliferative activity in BM. Thrombocytosis detected in the second groupcounterbalanced by ubiquitin in the third group. Erythrocyte’s count remained almost unchanged. One can assume that Platelet count increase in PB is associated with passage of both megakaryocytes and morphologically transformed erythroblasts. Further investigation with appliance of more sophisticated technics is necessary to evince feasibility of ubiquitin involvement in erythroblast/platelet transition. Probability of prevention of thrombocytosis by extracellular ubiquitin is high. More so as ubiquitins ability to moderate leukocyte regeneration picks was corroborated by our previous works.